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Forskolin augments the effects of calcitonin on cytoplasmic spreading of isolated rat osteoclasts and plasma calcium levels in the rat
Author(s) -
Nicholson G.C.,
Yumita S.,
Moseley J.M.,
Yates A.J.P.,
Martin T.J.
Publication year - 1988
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650030209
Subject(s) - forskolin , calcitonin , endocrinology , medicine , stimulation , calcium , chemistry , osteoclast , calcitonin receptor , biology , receptor , neuropeptide , calcitonin gene related peptide
Recently we have shown that forskolin acts synergistically with calcitonin on stimulation of cyclic AMP (cAMP) production in isolated rat osteoclasts and now show that forskolin also augments later physiological responses to calcitonin. The sensitivity of calcitonin inhibition of cytoplasmic spreading in isolated rat osteoclasts was increased 3.5‐fold by simultaneous treatment with 10 −7 M forskolin, which alone had no effect on the response. In weanling rats the threshold of the hypocalcemic response to salmon calcitonin was reduced fivefold by simultaneous treatment with 200 μg/kg forskolin i.v., which alone did not influence the plasma calcium. These results provide further evidence that calcitonin‐induced inhibition of osteoclast function is mediated by elevation of cAMP levels and suggest that forskolin could be used to augment the therapeutic effects of calcitonin.