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Retinole acid and glucocorticoids enhance the effect of 1,25‐dihydroxyvitamin D 3 on bone γ‐carboxyglutamic acid protein synthesis by rat osteosarcoma cells
Author(s) -
Nishimoto Satoru K.,
Salka Carl,
Nimni Marcel E.
Publication year - 1987
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650020614
Subject(s) - retinoic acid , medicine , endocrinology , secretion , calcitriol , stimulation , tretinoin , chemistry , triamcinolone acetonide , parathyroid hormone , biology , vitamin d and neurology , calcium , biochemistry , immunology , gene
Abstract Two 1,25‐dihydroxyvitamin D 3 ‐controlled parameters in the osteoblastlike osteosarcoma cell line ROS 17/2, bone γ‐carboxyglutamic acid‐containing protein (BGP) and collagen synthesis, were measured after pretreatments with either retinoic acid (RA), or triamcinolone acetate (TRM). RA and TRM both caused double the expected increase in BGP secretion at 16 hr after treatment with 1,25‐dihydroxy vitamin D 3 . Triamcinolone acetate concentrations of 10 −8 and 10 −9 M or 10 −6 M retinoic acid were effective in enhancing the 1,25‐dihydroxyvitamin D 3 stimulation of BGP secretion. Treatment with RA or TRM alone did not stimulate BGP secretion. RA alone had no effect on BGP secretion, while TRM inhibited BGP secretion. Collagen synthesis is inhibited by 1,25‐dihydroxyvitamin D 3 . Neither retinoic acid nor triamcinolone acetate enhanced the 1,25‐dihydroxyvitamin D 3 ‐mediated inhibition of collagen synthesis. Retinoic acid by itself inhibited collagen synthesis but did not change the 1,25 dihydroxyvitamin D 3 ‐mediated inhibition of collagen synthesis. Triamcinolone acetate by itself or together with 1,25‐dihydroxy vitamin D 3 increased collagen synthesis. We conclude that, although both triamcinolone acetate and retinoic acid increase the 1,25‐dihydroxy vitamin D 3 stimulation of BGP secretion by ROS 17/2 cells, they have different effects on the regulation of collagen production. Thus, although both hormones increase the 1,25‐dihydroxy vitamin D 3 receptor concentration in these cells, their actions are not mediated solely by this mechanism.