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J774A.1 macrophage cell line produces PDGF‐like and non‐PDGF‐like growth factors for bone cells
Author(s) -
Cheng S. L.,
Rifas L.,
Shen V.,
Tong B.,
Pierce G.,
Deuel T.,
Peck William A.
Publication year - 1987
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650020515
Subject(s) - platelet derived growth factor receptor , platelet derived growth factor , growth factor , microbiology and biotechnology , cell culture , cell growth , 3t3 cells , bone cell , biology , osteoblast , chemistry , receptor , biochemistry , transfection , in vitro , genetics
In light of evidence that macrophages participate in the local regulation of bone remodeling, we have examined the production of peptide stimulators of bone cell growth and specialization by the J774A.1 macrophage cell line. Cultured J774A.1 cells secrete growth‐promoting activities which have an affinity for heparin. The first partially purified materia), termed HEP I, appears to contain platelet‐derived growth factor (PDGF)‐like activity. It has a molecular weight of about 30,000 daltons, inhibits the binding of labeled PDGF to its receptors, reacts with polyclonal anti‐human PDGF antibody, and exhibits mitogenic activity for osteoblasts, which is partially blocked by anti‐PDGF antisera. Like PDGF, HEP I is active in a wide variety of mesenchyme‐derived cells, including osteoblasts, chondrocytes, smooth muscle cells, fibroblasts, 3T3 cells and NRK cells. The J774A.1 cells contain mRNA, which hybridizes to a v‐sis DNA probe, suggesting that they express the c‐sis gene, which contains the code for a PDGF‐like protein. The second factor, HEP II, has an approximate molecular weight of 20,000 daltons and possesses substantial mitogenic activity for osteoblasts, chondrocytes, and smooth muscle cells, but is not mitogenic for fibroblasts, 3T3 cells, and NRK cells. HEP II appears to be a unique bone cell mitogen, which is distinct from the growth factors presently known. Neither HEP I nor HEP II contained interleukin 1, a macrophage product known to promote bone resorption and perhaps the growth and activity of osteoblasts.