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Binding of calcitonin and calcitonin gene‐related peptide to calvarial cells and renal cortical membranes
Author(s) -
Seitz Patricia K.,
Thomas Mary L.,
Cooper Cary W.
Publication year - 1986
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.5650010109
Subject(s) - calcitonin gene related peptide , calcitonin , medicine , receptor , endocrinology , neuropeptide , chemistry , renal cortex , binding site , peptide , calcitonin receptor , biology , kidney , biochemistry
Binding of calcitonin (CT) and calcitonin gene‐related peptide (CGRP) to rat hemicalvariae and renal membranes was examined in an effort to determine whether CT and CGRP interact with the same bone cell binding site, and to see whether the binding pattern was similar for bone and renal cortex. Specific binding of 125 I‐salmon CT to rat calvariae was inhibited by unlabeled salmon, porcine, or human CT, but not by rat CGRP. Binding of 125 I‐rat CGRP to calvariae was inhibited by CGRP and high doses of salmon CT, but not by human or porcine CT. Binding of 125 I‐salmon CT to renal membranes was inhibited by unlabeled salmon CT or rat CGRP, but no specific binding of 125 I‐rat CGRP could be detected. The results suggest that separate bone cell receptors for CT and CGRP exist and that CGRP can interact with renal receptors for CT.