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GATA4 Is Essential for Bone Mineralization via ERα and TGFβ/BMP Pathways
Author(s) -
Güemes Miriam,
Garcia Alejandro J,
Rigueur Diana,
Runke Stephanie,
Wang Weiguang,
Zhao Gexin,
Mayorga Victor Hugo,
Atti Elisa,
Tetradis Sotirios,
Péault Bruno,
Lyons Karen,
MirandaCarboni Gustavo A,
Krum Susan A
Publication year - 2014
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.2296
Subject(s) - mineralization (soil science) , bone morphogenetic protein , signal transduction , transforming growth factor , bone morphogenetic protein 2 , microbiology and biotechnology , chemistry , cancer research , endocrinology , medicine , biology , biochemistry , gene , in vitro , organic chemistry , nitrogen
Osteoporosis is a disease characterized by low bone mass, leading to an increased risk of fragility fractures. GATA4 is a zinc‐finger transcription factor that is important in several tissues, such as the heart and intestines, and has recently been shown to be a pioneer factor for estrogen receptor alpha (ERα) in osteoblast‐like cells. Herein, we demonstrate that GATA4 is necessary for estrogen‐mediated transcription and estrogen‐independent mineralization in vitro. In vivo deletion of GATA4, driven by Cre‐recombinase in osteoblasts, results in perinatal lethality, decreased trabecular bone properties, and abnormal bone development. Microarray analysis revealed GATA4 suppression of TGFβ signaling, necessary for osteoblast progenitor maintenance, and concomitant activation of BMP signaling, necessary for mineralization. Indeed, pSMAD1/5/8 signaling, downstream of BMP signaling, is decreased in the trabecular region of conditional knockout femurs, and pSMAD2/3, downstream of TGFβ signaling, is increased in the same region. Together, these experiments demonstrate the necessity of GATA4 in osteoblasts. Understanding the role of GATA4 to regulate the tissue specificity of estrogen‐mediated osteoblast gene regulation and estrogen‐independent bone differentiation may help to develop therapies for postmenopausal osteoporosis. © 2014 American Society for Bone and Mineral Research.