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Overestimation of the 25(OH)D serum concentration with the automated IDS EIA kit
Author(s) -
Cavalier Etienne,
Huberty Véronique,
Cormier Catherine,
Souberbielle JeanClaude
Publication year - 2011
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.190
Subject(s) - computer science , medicine
We have recently observed an increasing number of patients presenting very high serum levels of 25‐hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different “open” automated platforms. We performed a comparison between the IDS EIA kit adapted on two different “open”automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50–60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit. © 2011 American Society for Bone and Mineral Research.