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Runx1 dose‐dependently regulates endochondral ossification during skeletal development and fracture healing
Author(s) -
Soung Do Y,
Talebian Laleh,
Matheny Christina J,
Guzzo Rosa,
Speck Maren E,
Lieberman Jay R,
Speck Nancy A,
Drissi Hicham
Publication year - 2012
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.1601
Subject(s) - chondrogenesis , endochondral ossification , runx1 , runx2 , mesenchymal stem cell , biology , cartilage , progenitor cell , embryonic stem cell , bone healing , sox9 , microbiology and biotechnology , endocrinology , anatomy , medicine , stem cell , osteoblast , haematopoiesis , transcription factor , genetics , in vitro , gene
Abstract Runx1 is expressed in skeletal elements, but its role in fracture repair has not been analyzed. We created mice with a hypomorphic Runx1 allele (Runx1 L148A ) and generated Runx1 L148A/− mice in which >50% of Runx1 activity was abrogated. Runx1 L148A/− mice were viable but runted. Their growth plates had extended proliferating and hypertrophic zones, and the percentages of Sox9‐, Runx2‐, and Runx3‐positive cells were decreased. Femoral fracture experiments revealed delayed cartilaginous callus formation, and the expression of chondrogenic markers was decreased. Conditional ablation of Runx1 in the mesenchymal progenitor cells of the limb with Prx1‐Cre conferred no obvious limb phenotype; however, cartilaginous callus formation was delayed following fracture. Embryonic limb bud–derived mesenchymal cells showed delayed chondrogenesis when the Runx1 allele was deleted ex vivo with adenoviral‐expressed Cre. Collectively, our data suggest that Runx1 is required for commitment and differentiation of chondroprogenitor cells into the chondrogenic lineage. © 2012 American Society for Bone and Mineral Research.