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Biomechanical stimulation of osteoblast gene expression requires phosphorylation of the RUNX2 transcription factor
Author(s) -
Li Yan,
Ge Chunxi,
Long Jason P,
Begun Dana L,
Rodriguez Jose A,
Goldstein Steven A,
Franceschi Renny T
Publication year - 2012
Publication title -
journal of bone and mineral research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.882
H-Index - 241
eISSN - 1523-4681
pISSN - 0884-0431
DOI - 10.1002/jbmr.1574
Subject(s) - runx2 , mapk/erk pathway , osteoblast , microbiology and biotechnology , phosphorylation , transcription factor , kinase , gene expression , biology , signal transduction , chromatin , chemistry , gene , genetics , in vitro
Bone can adapt its structure in response to mechanical stimuli. At the cellular level, this involves changes in chromatin organization, gene expression, and differentiation, but the underlying mechanisms are poorly understood. Here we report on the involvement of RUNX2, a bone‐related transcription factor, in this process. Fluid flow shear stress loading of preosteoblasts stimulated translocation of extracellular signal‐regulated kinase (ERK)/mitogen‐activated protein kinase (MAPK) to the nucleus where it phosphorylated RUNX2 on the chromatin of target genes, and increased histone acetylation and gene expression. MAPK signaling and two RUNX2 phosphoacceptor sites, S301 and S319, were critical for this response. Similarly, in vivo loading of mouse ulnae dramatically increased ERK and RUNX2 phosphorylation as well as expression of osteoblast‐related genes. These findings establish ERK/MAPK‐mediated phosphorylation of RUNX2 as a critical step in the response of preosteoblasts to dynamic loading and define a novel mechanism to explain how mechanical signals induce gene expression in bone. © 2012 American Society for Bone and Mineral Research.