Open AccessSolid‐State Nuclear Magnetic Resonance (SSNMR) Characterization of Osteoblasts From Mesenchymal Stromal Cell Differentiation to Osteoblast Mineralization
Author(s) -
Lin JingYu,
Sun MingHui,
Zhang Jing,
Hu Meng,
Zeng YuTeng,
Yi QianQian,
Wang Jian,
Bai Yun,
Zhang Yifeng,
Lu JunXia
Publication year - 2022
Publication title -
jbmr plus
Language(s) - English
Resource type - Journals
ISSN - 2473-4039
DOI - 10.1002/jbm4.10662
Subject(s) - mesenchymal stem cell , osteoblast , mineralization (soil science) , chemistry , extracellular matrix , stromal cell , cellular differentiation , microbiology and biotechnology , cell , in vitro , biochemistry , biology , cancer research , organic chemistry , gene , nitrogen
Solid‐state nuclear magnetic resonance (SSNMR), a technique capable of studying solid or semisolid biological samples, was first applied to study the cell differentiation and mineralization using the whole‐cell sample. Mesenchymal stromal cells (MSCs) with multipotent differentiation capacity were induced to differentiate into osteoblasts. The whole differentiation process, osteoblast mineralization and the mineral maturation, was investigated using SSNMR, providing intact, atomic level information on the cellular mineral structural transformation. Our research indicated the extent of osteoblast mineralization could vary significantly for different cell populations whereas the difference was not easily shown by other means of characterization. The SSNMR spectra revealed hydroxylapatite (or hydroxyapatite [HAP]) formation around 2 to 4 weeks after osteogenic induction for MSCs with a high differentiation potency. The early mineral phase deposit before HAP formation contained a high amount of HPO 4 2− . The structures of minerals in the extracellular matrix (ECM) of osteoblasts could evolve for a period of time, even after the incubation of cells has been stopped. This observation was only possible by studying the sample in an intact state, where ECM was not disturbed. These findings improved our understanding of MSCs, which had wide applications in bone regeneration and tissue engineering. Meanwhile, this work demonstrated the advantage of studying these cellular systems as a whole without any mineral extraction, which had been largely overlooked. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.