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Lactobacillus cell‐free supernatant as a novel bioagent and biosurfactant against Pseudomonas aeruginosa in the prevention and treatment of orthopedic implant infection
Author(s) -
Jeyanathan Augustina,
Ramalhete Rita,
Blunn Gordon,
Gibbs Hannah,
Pumilia Cyrus Anthony,
Meckmongkol Teerin,
Lovejoy John,
Coathup Melanie J.
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34821
Subject(s) - biofilm , lactobacillus fermentum , lactobacillus plantarum , microbiology and biotechnology , lactobacillus , lactobacillus acidophilus , probiotic , pseudomonas aeruginosa , chemistry , colony forming unit , bacteria , biology , food science , lactic acid , fermentation , genetics
The hypothesis was that probiotic Lactobacillus species (spp.) or their cell‐free supernatant (CFS) are effective in inhibiting (a) planktonic growth of Pseudomonas aeruginosa (PA), (b) its adhesion to a Ti6Al4V‐alloy surface, and (c) in dispersing biofilm once formed. (a) A planktonic co‐culture containing PA(10 4 colony‐forming unit [CFU]/ml) was combined with either Lactobacillus acidophilus , Lactobacillus plantarum (LP), or Lactobacillus fermentum (LF) at a suspension of 10 4 (1:1) or 10 8 CFU/ml (1:2). Lactobacillus and PA CFUs were then quantified. (b) Ti‐6Al‐4V discs were inoculated with PA followed by supplementation with CFS and adherent PA quantified. (c) Biofilm covered discs were supplemented with Lactobacillus CFS and remaining PA activity quantified. Results showed that whole‐cell cultures were ineffective in preventing PA growth; however, the addition of CFS resulted in a 99.99 ± 0.003% reduction in adherent PA in all Lactobacillus groups ( p < .05 in all groups) with no viable PA growth measured in the LF and LP groups. Following PA biofilm formation, CFS resulted in a significant reduction in PA activity in all Lactobacillus groups ( p ≤ .05 in all groups) with a 29.75 ± 15.98% increase measured in control samples. Supplementation with CFS demonstrated antiadhesive, antibiofilm, and toxic properties to PA.