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Micropatterning of polymer substrates for cell culture
Author(s) -
Yu Suzhu,
Liu Dan,
Wang Tianyi,
Lee Yi Zhen,
Wong Joshua Cheng Ning,
Song Xu
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34811
Subject(s) - micropatterning , petri dish , polydimethylsiloxane , materials science , thermosetting polymer , silicone rubber , substrate (aquarium) , polystyrene , polymer , silicone , adhesion , thermoplastic , photoresist , composite material , nanotechnology , layer (electronics) , oceanography , genetics , geology , biology
The cell microenvironment such as substrate topology plays an important role in biological processes. In this study, microgrooves were successfully produced on surfaces of both thermoplastic and thermoset polymers using cost‐effective techniques for mass production. The micropatterning of thermoplastic polystyrene (PS) petri dish was accomplished efficiently using an in‐house developed low‐cost hot embossing system. The high replication fidelity of the microgroove with depth and width of 2 μm and spacing of 2 μm was achieved by using silicone rubber as a soft counter mold. This patterned petri dish subsequently served as the cast to replicate the micropattern onto thermoset polydimethylsiloxane (PDMS). It was found that the micropattern increased the hydrophobicity of both PS and PDMS surfaces. The effect of the substrate micropattern on cellular behaviors was preliminarily investigated with untreated and treated PS petri dish as well as PDMS. The results show that the micropattern significantly improved cell adhesion and proliferation for cells cultured on untreated PS petri dish and PDMS substrates. Moreover, the micropattern induced obvious cell alignment along the microgrooves for culturing on all substrates which were studied.