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Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate
Author(s) -
Teixeira Ana B. V.,
Moreira Natália C. S.,
Takahashi Catarina S.,
Schiavon Marco A.,
Alves Oswaldo L.,
Reis Andréa C.
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34798
Subject(s) - cytotoxicity , viability assay , cytotoxic t cell , genotoxicity , apoptosis , vanadate , fibroblast , chemistry , pharmacology , microbiology and biotechnology , in vitro , nuclear chemistry , biochemistry , toxicity , medicine , biology , organic chemistry
The cytotoxic and genotoxic effects of commercial endodontic sealers (AH Plus, Sealer 26 and Endomethasone N) incorporated with nanostructured silver vanadate decorated with silver nanoparticles (AgVO 3 – at concentrations 2.5, 5, and 10%) on human gingival fibroblast (HGF), and the silver (Ag + ) and vanadium (V 4+ /V 5+ ) ions release were evaluated. Cytotoxicity, cell death, and genotoxicity tests were carried out with extract samples of 24‐hr and 7‐days. The release of Ag + and V 4+ /V 5+ was evaluated. Cytotoxicity in HGF was caused by AH Plus (AP) with 5 and 10% of AgVO 3 (83.84 and 67.49% cell viability, respectively) with 24‐hr extract ( p < 0.05), as well as all concentrations of AP with 7‐days extract ( p < 0.05 –AP 0% = 73.17%; AP 2.5% = 75.07%; AP 5% = 70.62%; AP 10% = 68.46% cell viability). The commercial sealers Sealer 26 (S26) and Endomethasone N (EN) were cytotoxic ( p < 0.05 – S26 0% = 34.81%; EN 0% = 20.99% cell viability with 7‐days extract). AP 10% with 7‐days extract induced 32% apoptotic cells in HGF ( p < 0.05). Genotoxic effect was not observed. The AP groups released more Ag + , while S26 and EN released more V 4+ /V 5+ in 24 hr. The Ag + can be cytotoxic. In conclusion, the cytotoxicity caused to HGF can be attributed by the commercial sealers and enhanced by incorporation of AgVO 3 , was not observed genotoxic effect, and apoptosis was induced only by AH Plus 10% 7‐days extract. Ag + can influence cell viability.