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Development of fibronectin‐loaded nanofiber scaffolds for guided pulp tissue regeneration
Author(s) -
Leite Maria Luísa,
Soares Diana Gabriela,
Anovazzi Giovana,
Mendes Soares Igor Paulino,
Hebling Josimeri,
Souza Costa Carlos Alberto
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34785
Subject(s) - materials science , nanofiber , fibronectin , regeneration (biology) , electrospinning , polycaprolactone , pulp (tooth) , cell adhesion , biomaterial , adhesion , biomedical engineering , biophysics , chemistry , polymer , extracellular matrix , nanotechnology , microbiology and biotechnology , composite material , dentistry , biochemistry , biology , medicine
Fibronectin (FN)‐loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) and aligned (NA) nanofiber scaffolds of polycaprolactone (PCL) were obtained by electrospinning technique and their biological properties were evaluated. The best formulations of NR and NA were loaded with 0, 5, or 10 μg/ml of FN and their bioactivity was assessed. Finally, FN‐loaded NR and NA tubular scaffolds were prepared and their chemotactic potential was analyzed using an in vitro model to mimic the pulp regeneration of teeth with incomplete root formation. All scaffolds tested were cytocompatible. However, NR and NA based on 10% PCL promoted the highest hAPCs proliferation, adhesion and spreading. Polygonal and elongated cells were observed on NR and NA, respectively. The higher the concentration of FN added to the scaffolds, greater cell migration, viability, proliferation, adhesion and spreading, as well as collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1). In addition, tubular scaffolds with NA loaded with FN (10 μg/ml) showed the highest chemotactic potential on hAPCs. It was concluded that FN‐loaded NA scaffolds may be an interesting biomaterial to promote hAPCs‐mediated pulp regeneration of endodontically compromised teeth with incomplete root formation.