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Roughness and wettability of titanium implant surfaces modify the salivary pellicle composition
Author(s) -
MartínezHernández Miryam,
Hannig Matthias,
GarcíaPérez Victor I.,
OlivaresNavarrete Rene,
FecherTrost Claudia,
AlmaguerFlores Argelia
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34766
Subject(s) - wetting , titanium , contact angle , bicinchoninic acid assay , protein adsorption , chemistry , adsorption , surface finish , proteome , surface roughness , mass spectrometry , nuclear chemistry , materials science , electrospray , chromatography , biochemistry , composite material , organic chemistry
This study reports the differences in the protein composition of salivary pellicles formed under in situ conditions on two Titanium (Ti) surfaces, with different roughness and wettability. Smooth pretreatment Ti surfaces (Ti‐PT) with an average roughness (Ra) of 0.45 μm and a water contact angle (WCA) of 92.4°, as well as a more rough sandblasted, large grit, acid‐etched treatment Ti surfaces (Ti‐SLA) with a Ra of 3.3 μm and WCA of 131.8°, were tested. The salivary pellicles were quantitatively analyzed by bicinchoninic acid assays, and the protein identification was performed by Nano‐LC–MS/MS (nano mass spectrometry). Protein levels of 2.5, and 9.1 μg/ml were quantified from the detached salivary pellicle formed on the Ti‐PT and Ti‐SLA surfaces, respectively. Using Nano‐LC–MS/MS, a total of 597 proteins were identified on all the substrates tested; 43 proteins were identified only on the Ti‐PT, and 226 proteins were adsorbed solely on the Ti‐SLA substrates. The physicochemical characteristics of the Ti implant surfaces modified the amount and the identity of the salivary proteome of the pellicles formed, confirming the high selectivity of the protein pellicle formed on a surface once is exposed in the oral cavity.