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Quantitative assessment of dentine mineralization and tubule occlusion by NovaMin and stannous fluoride using serial block face scanning electron microscopy
Author(s) -
Mahmoodi Behrad,
Goggin Patricia,
Fowler Christabel,
Cook Richard B.
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34737
Subject(s) - scanning electron microscope , dentine hypersensitivity , fluoride , chemistry , dentistry , occlusion , materials science , biomedical engineering , composite material , inorganic chemistry , medicine , surgery
Dentine hypersensitivity (DH) is one of the most common dental conditions affecting most adults during their lifetime. Tubule occlusion is a widely accepted method for treating DH. Current in‐vitro techniques such as focused ion beam, scanning electron microscopy (SEM), or hydraulic conductance that are used to determine tubule occlusion do not provide the depth of occlusion, are time‐consuming, expensive and the volume of dentine tested is limited. The presented study aimed to assess the ability of serial block‐face SEM (SBF‐SEM) to section dentine, to quantify the number of occluded tubules including the depth of penetration by NovaMin and stannous fluoride (SnF 2 ) and to compare mineral density between the control and treated dentine. Results demonstrated that NovaMin provided a better occlusion with 100% of the tubules blocked at the surface compared to 83% for SnF 2 . The grayscale value (230.42) was significantly higher ( p ≤ 0.05) after treatment with NovaMin compared to SnF 2 (222.06) and the control (196.37), indicating increased mineral density and dentine mineralization. SBF‐SEM has the potential to be used for large volume analysis of bone‐like materials at high resolution with minimal sample preparation over a short period. It can be significantly useful in the development and research of new biomaterials.