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Bioactivity and antibacterial activity of strontium and silver ion releasing titanium
Author(s) -
Masamoto Kazutaka,
Fujibayashi Shunsuke,
Yamaguchi Seiji,
Otsuki Bungo,
Okuzu Yaichiro,
Kawata Tomotoshi,
Goto Koji,
Shimizu Takayoshi,
Shimizu Yu,
Kawai Toshiyuki,
Hayashi Makoto,
Morizane Kazuaki,
Imamura Masashi,
Ikeda Norimasa,
Takaoka Yusuke,
Matsuda Shuichi
Publication year - 2021
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34695
Subject(s) - titanium , in vivo , strontium , nuclear chemistry , materials science , antibacterial activity , simulated body fluid , biomedical engineering , chemistry , metallurgy , medicine , biology , bacteria , composite material , scanning electron microscope , genetics , microbiology and biotechnology , organic chemistry
To overcome problems associated with loosening of orthopedic implants and surgical site infections, we developed a novel, titanium (Ti)‐based material that releases both strontium and silver ions (CaSrAg–Ti) based on alkali‐and‐heat treatment. The results of commercially pure Ti (cp–Ti), Ti that releases Sr ions only (CaSr–Ti), and the novel CaSrAg–Ti material were compared. Mechanical tests were performed to evaluate the in vivo bonding properties of CaSrAg–Ti and the bone‐implant contact (BIC) ratio in histological specimens was determined at 4 and 8 weeks after implantation in a rat femur. Also, the in vitro antibacterial activities of this material against methicillin‐susceptible Staphylococcus aureus (MSSA) were evaluated after a 24 h incubation period by assaying colony‐forming units. In addition, antibacterial activities were evaluated in vivo at 7 days after implantation in a rat subcutaneous pocket model. There was direct contact between the bone and CaSrAg–Ti in histological specimens and no apparent signs of argyrosis in any rat. The bone‐bonding strength and the BIC ratio were increased by 2.7‐ and 2.3‐fold for CaSrAg–Ti vs. cp‐Ti at 4 weeks and 2.2‐ and 2.0‐fold at 8 weeks, respectively. As compared with cp‐Ti, the number of viable MSSA remaining on CaSrAg–Ti was reduced by 100 ± 0% in vitro and 94.2 ± 6.9% in vivo. Ti that releases Sr and Ag ions is a promising material that exhibits both bone‐bonding properties and anti‐MSSA activities.