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Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro
Author(s) -
Ding Longjiang,
Han Sili,
Peng Xiu,
Wang Kun,
Zheng Sainan,
Li Haoran,
Niu Yumei,
Li Wei,
Zhang Linglin
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34663
Subject(s) - enamel paint , remineralisation , materials science , crystallization , polarized light microscopy , in vitro , tooth remineralization , apatite , transmission electron microscopy , scanning electron microscope , biomedical engineering , nuclear chemistry , biophysics , dentistry , chemical engineering , chemistry , mineralogy , biochemistry , nanotechnology , composite material , medicine , optics , biology , engineering , physics
With the gradual discovery of functional domains in natural proteins, several biologically inspired peptides have been designed for use as biomaterials for hard tissue regeneration and repair. In this study, we designed a tuftelin‐derived peptide (TDP) and tested its effects on hydroxyapatite crystallization and remineralization of initial enamel carious lesions in vitro. Using circular dichroism spectroscopy, we found that TDP contained 36.1% β‐sheets and β‐turns, which could be influenced by calcium ions. We verified the ability of TDP to crystallize hydroxyapatite using transmission electron microscopy and its ability to bind to the enamel surface and hydroxyapatite using confocal laser scanning microscopy and Langmuir adsorption isotherms ( K = 881.56, N = 1.41 × 10 −5 ). Artificial enamel lesions were generated on human enamel blocks and subjected to a 12‐day pH cycling model and were treated with 25 μM TDP, 1 g/L sodium fluoride (NaF), or deionized water. We analyzed the results of remineralization by surface microhardness testing, polarized light microscopy, and transverse microradiography. The TDP group showed significantly higher surface microhardness recovery (49.21 ± 1.66%), shallower lesions (34.89 ± 4.05 μm), and less mineral loss (871.33 ± 81.49 vol%·μm) after pH cycling than the deionized water group ( p < .05). There were no significant differences between the TDP and NaF groups. Our experiment indicated that TDP could regulate hydroxyapatite crystallization and promote remineralization of enamel caries in vitro.