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Physical and biological properties of blend‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N , N ‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study
Author(s) -
Zafari Mahdi,
Mansouri Milad,
Omidghaemi Shadi,
Yazdani Amid,
Pourmotabed Samiramis,
Hasanpour Dehkordi Ali,
Nosrati Hamed,
Validi Majid,
Sharifi Esmaeel
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34636
Subject(s) - polycaprolactone , wound healing , electrospinning , chitosan , nanofiber , materials science , in vivo , nuclear chemistry , chemistry , composite material , organic chemistry , surgery , medicine , polymer , biology , microbiology and biotechnology
Dual‐pump electrospinning of antibacterial N‐decyl‐N, N‐dimethyl‐1‐decanaminium‐chloride (DDAC)‐loaded polycaprolactone (PCL) nanofibers, and chitosan (CS)/polyethylene‐oxide (PEO)‐based wound dressings with hydrophilic and hydrophobic properties to eliminate and absorb pathogenic bacteria from wound surface besides antibacterial action and to support wound healing and accelerate its process. Physicochemical properties of the prepared nanofibrous mat as well as antibacterial, cytotoxicity, and cell compatibility were studied. The full‐thickness excisional wound healing properties up to 3 weeks using hematoxylin and eosin and Masson‐trichrome staining were investigated. Addition of DDAC to CS/PEO‐PCL mats decreased the diameter of the nanofibers, which is a crucial property for wound healing as large surface area per volume ratio of nanofibers, in addition to proper cell adhesion, increases loading of DDAC in mats and leads to increased cell viability and eliminating Gram‐positive bacteria at in vitro studies. In vivo studies showed DDAC‐loaded CS/PEO‐PCL mats increased epithelialization and angiogenesis and decreased the inflammation according to histological results. We demonstrated that hydrophobic PCL/DDAC mats, besides antibacterial properties of DDAC, absorbed and eliminated the hydrophobic pathological microorganisms, whereas the hydrophilic nanofibers consisted of CS/PEO, increased the cell adhesion and proliferation due to positive charge of CS. Finally, we were able to increase the wound healing quality by using multifunctional wound dressing. CS/PEO‐PCL containing 8 wt % of DDAC nanofibrous mats is promising as a wound dressing for wound management due to the favorable interactions between the pathogenic bacteria and PCL/CS‐based wound dressing.