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High porous electrospun poly(ε‐caprolactone)/gelatin/ MgO scaffolds preseeded with endometrial stem cells promote tissue regeneration in full‐thickness skin wounds: An in vivo study
Author(s) -
Ababzadeh Shima,
Farzin Ali,
Goodarzi Arash,
Karimi Roya,
Sagharjoghi Farahani Morteza,
Eslami Farsani Mohsen,
Gharibzad Kamelia,
Zahiri Maria,
Ai Jafar
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34626
Subject(s) - gelatin , wound healing , in vivo , caprolactone , electrospinning , materials science , biomedical engineering , regeneration (biology) , tissue engineering , nanofiber , polylactic acid , chemistry , composite material , surgery , polymer , medicine , biochemistry , microbiology and biotechnology , copolymer , biology
In the current study, electrospun poly(ε‐caprolactone)‐gelatin (PCL‐Gel) fibrous scaffolds containing magnesium oxide (MgO) particles and preseeded with human endometrial stem cells (hEnSCs) were developed to use as wound care material in skin tissue engineering applications. Electrospun fibers were fabricated using PCL‐Gel (1:1 [wt/wt]) with different concentrations of MgO particles (1, 2, and 4 wt%). The fibrous scaffolds were evaluated regarding their microstructure, mechanical properties, surface wettability, and in vitro and in vivo performances. The full‐thickness excisional wound model was used to evaluate the in vivo wound healing ability of the fabricated scaffolds. Our findings confirmed that the wounds covered with PCL‐Gel fibrous scaffolds containing 2 wt% MgO and preseeded with hEnSCs have nearly 79% wound closure ability while sterile gauze showed 11% of wound size reduction. Our results can be employed for biomaterials aimed at the healing of full‐thickness skin wounds.