Premium
A novel photostable near‐infrared‐to‐near‐infrared fluorescent nanoparticle for in vivo imaging
Author(s) -
Fan Qi,
Cui Xiaoxia,
Wang Quan,
Gao Peng,
Shi Shengjia,
Wen Weihua,
Guo Haitao,
Xu Yantao,
Peng Bo
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34622
Subject(s) - materials science , photothermal therapy , fluorescence , biocompatibility , nanoparticle , biodistribution , fluorescence lifetime imaging microscopy , near infrared spectroscopy , in vivo , laser , preclinical imaging , infrared , nanotechnology , optics , physics , microbiology and biotechnology , metallurgy , biology
Water‐soluble K 5 HoLi 2 F 10 (KHLF) nanoprobes with the excitation and emission both in the near‐infrared ( NIR) region were developed and first demonstrated for in vivo imaging of living mice. The PEG 400 coating endows the nanoprobes with good water solubility and biocompatibility. Doping with Ho 3+ ions is capable of emitting NIR fluorescence with two peaks centered, respectively, at 887 and 1,180 nm once excited by a 808 nm laser; meanwhile, it also possess good photothermal conversion performance. The KHLF matrix with specifically structure of large ion‐distance and low photon energy imparts the nanoprobes low quenching effect and excellent photostability (fluorescence decrease <5% upon 120 min illumination of 808 nm continuous laser with a power density of 1 W/cm 2 ). The nanoparticles ( NPs) were tested for in vitro bioimaging with living mice. The results show the NPs have low biotoxicity, rapid metabolism, normal biodistribution, together with the photothermal imaging performance and a high‐contrast fluorescence images (signal‐to‐background ratio of 14:1). The superior performances of these nanoprobes in vivo imaging of mice proclaim the great potential of this type of probe for high‐contrast imaging and photothermal treatment in practical applications.