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Designing laser‐modified surface structures on titanium alloy custom medical implants using a hybrid manufacturing technology
Author(s) -
Komorowski Piotr,
Sokołowska Paulina,
Siatkowska Małgorzata,
Elgalal Marcin,
Rosowski Marcin,
Makowski Krzysztof,
Lipińska Lidia,
Leszczewicz Martyna,
Styczyński Andrzej,
Fogel Kasper,
Walkowiak Bogdan
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34521
Subject(s) - osseointegration , adhesion , materials science , titanium alloy , surface modification , biomedical engineering , viability assay , titanium , cell adhesion , implant , nanotechnology , chemical engineering , alloy , chemistry , metallurgy , cell , composite material , medicine , surgery , engineering , biochemistry
The hybrid technology combines an efficient material‐removal process and implant surface treatment by the laser reducing time of manufacture process compared to currently used machining technologies. It also permits precise structuring of the implant material surface. Six structures of the Ti6Al4V ELI surface were designed and studied how the structure topography prepared with the hybrid technology affected the Escherichia coli adhesion to the surface and viability, as well as the growth, adhesion, and viability of human osteogenic Saos‐2 cells cultured on the investigated surfaces. Results have confirmed that the microtopography of medical titanium alloy plays a beneficial role in bacterial adhesion and viability (number of bacteria found on reference surface: [5.9 ± 0.44] × 10 6 CFU/ml, sample no. 3: [8.8 ± 0.93] × 10 4 CFU/ml, and sample no. 5: [1.2 ± 0.23] × 10 7 CFU/ml; CFU ‐ Colony Forming Unit). All tested structured surfaces enabled good cell attachment and proliferation of Saos‐2 cells (viability of Saos‐2 cells [% of control] for reference surface: 81.93%; sample no. 3: 75% and sample no. 5: 100%). Transcriptome analysis of genes commonly expressed in the process of osseointegration demonstrated that the use of hybrid technology allows designing structures that enhance osseointegration but it should be coupled with other methods of preventing bacterial growth, or with a different strategy to limit microbial colonization with the satisfactory osseointegration potential.