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Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone
Author(s) -
Ressler Antonia,
Cvetnić Matija,
Antunović Maja,
Marijanović Inga,
Ivanković Marica,
Ivanković Hrvoje
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34515
Subject(s) - octacalcium phosphate , strontium , calcium , amorphous calcium phosphate , simulated body fluid , nuclear chemistry , chemistry , phosphate , biomineralization , calcite , hydroxyapatites , calcium carbonate , mineralogy , apatite , chemical engineering , biochemistry , organic chemistry , engineering
Abstract Biomimetic triphasic strontium‐substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO 3 , (NH 2 ) 2 COH 3 PO 4 , and Sr(NO 3 ) 2 as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X‐ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β‐tricalcium phosphate (β‐TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β‐TCP. Sr 2+ occupy the Ca(1) site in HA, Ca(3,4,7,8) sites in OCP and Ca(1,2,3,4) sites in β‐TCP. Along with Sr 2+ substitution, presence of Mg 2+ and Na + ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture.