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Temperature‐dependent mucosal permeation kinetics of stigmasterol microspheres: In vivo mice model antioral candidiasis study
Author(s) -
Niyogi Partha,
Pattnaik Snigdha,
Maharana Laxmidhar,
Mohapatra Rajaram,
Haldar Saikat
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34510
Subject(s) - permeation , chromatography , in vivo , chemistry , kinetics , penetration (warfare) , glutaraldehyde , materials science , membrane , biochemistry , physics , microbiology and biotechnology , quantum mechanics , biology , operations research , engineering
Abstract Evaluation of mucosal permeation of stigmasterol from the glutaraldehyde cross linked chitosan microspheres at increasing experimental temperatures was performed. The activation energy of permeation, partition, and diffusion were estimated to understand the permeation kinetic with respect to the temperature. The formulation depicting least activation energy possessed the increased permeation thresholds of drug at the site of application. The encapsulation efficacy and mucoadhesive strength were found to be directly proportional to the polymer‐emulsifier ratio. Decreased intensity in crystallography directed the molecular dispersion of microencapsulated drug. The depleted enthalpic phase transition in thermogram affirmed the stigmasterol encapsulation. The sphericity and the size of microspheres were determined by scanning electron photo micrograph. The in vivo quantification of oral Candida infection with different statistical approach and histopathological observation of infected tongue of mice on treatment with the stigmasterol encapsulated microspheres showed significant anti oral candidiasis activity by reduction of fungal colony count and recovery of papillae, reorganization of basal cell layer and newly formed papillae during 21–28 days of treatment.