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In vitro and in vivo osteogenic potential of niobium‐doped 45S5 bioactive glass: A comparative study
Author(s) -
Lopes João H.,
Souza Lucas P.,
Domingues Juliana A.,
Ferreira Filipe V.,
Alencar Hausen Moema,
Camilli José A.,
Martin Richard A.,
Rezende Duek Eliana A.,
Mazali Italo O.,
Bertran Celso A.
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34486
Subject(s) - bioactive glass , apatite , niobium oxide , materials science , in vivo , simulated body fluid , magic angle spinning , nuclear chemistry , niobium , inductively coupled plasma , solubility , analytical chemistry (journal) , chemical engineering , mineralogy , chemistry , composite material , nuclear magnetic resonance spectroscopy , chromatography , metallurgy , stereochemistry , organic chemistry , microbiology and biotechnology , plasma , physics , quantum mechanics , engineering , biology
In vitro and in vivo experiments were undertaken to evaluate the solubility, apatite‐forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb‐containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP‐OES) revealed that the rate at which Na, Ca, Si, P, and Nb species are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic resonance spectroscopy. Results showed that the bioactive glasses: Bioglass 45S5 (BG45S5) and 1 mol%‐Nb‐containing‐bioactive glass (BGSN1) were able to grow apatite layer on their surfaces within 3 h, while glasses with higher concentrations of Nb 2 O 5 (2.5 and 5 mol%) took at least 12 h. Nb‐substituted glasses were shown to be compatible with bone marrow‐derived mesenchymal stem cells (BMMSCs). Moreover, the bioactive glass with 1 mol% Nb 2 O 5 significantly enhanced cell proliferation after 4 days of treatment. Concentrations of 1 and 2.5 mol% Nb 2 O 5 stimulated osteogenic differentiation of BMMSCs after 21 days of treatment. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their osteoconductivity and osteostimulation. Two morphometric parameters were analyzed: (a) thickness of new‐formed bone layer and (b) area of new‐formed subperiostal bone. Results showed that BGNb bioactive glass is osteoconductive and osteostimulative. Therefore, these results indicate that Nb‐substituted glass is suitable for biomedical applications.