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In situ forming gelatin/hyaluronic acid hydrogel for tissue sealing and hemostasis
Author(s) -
Luo JingWan,
Liu Chang,
Wu JingHeng,
Zhao DanHui,
Lin LongXiang,
Fan HaiMing,
Sun YuLong
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34433
Subject(s) - self healing hydrogels , gelatin , materials science , hyaluronic acid , biocompatibility , fibrin glue , fibrin , hemostat , biomedical engineering , sealant , hemostatic agent , swelling , tissue adhesion , adhesion , hemostasis , polymer chemistry , composite material , chemistry , surgery , medicine , biochemistry , anatomy , immunology , metallurgy
Fibrin glue has been widely used as a surgical sealing and hemostatic agent. Its application is restricted due to poor tissue adhesion and low mechanical strength. To develop better tissue sealant and hemostatic agent, this study prepared the injectable hydrogels by chemically cross‐linking gelatin (G) with or without hyaluronic acid (HA) in situ at a mild condition. The rheological analysis, Fourier transform infrared spectroscopy, swelling, proteolytic degradation, biocompatibility, tissue sealing, and hemostatic ability of the hydrogels were investigated. It was found that the chemical cross‐linking rapidly formed in both self‐crosslinking gelatin (sc‐G) and gelatin/hyaluronate acid (G/HA) hydrogels. The hydrogels could be degraded by trypsin and had a desirable biocompatibility. The tissue sealing ability of the hydrogels was superior to fibrin glue. Furthermore, the G/HA hydrogel had similar hemostatic performance as fibrin glue, and was better than that of gelatin hydrogel. The results in the study indicated that the G/HA hydrogel could be used in clinic as a tissue sealant or surgical hemostat.