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Trilayer scaffold with cardiosphere‐derived cells for heart valve tissue engineering
Author(s) -
Chen Qi,
Bruyneel Arne,
Carr Carolyn,
Czernuszka Jan
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34427
Subject(s) - scaffold , materials science , tissue engineering , heart valve , elastin , biomedical engineering , native tissue , curvature , extracellular matrix , bending , biophysics , composite material , microbiology and biotechnology , biology , geometry , medicine , genetics , mathematics , cardiology
Natural polymers collagen, glycosaminoglycans, and elastin are promising candidate materials for heart valve tissue engineering scaffolds. This work produced trilayer scaffolds that resembled the layered structures of the extracellular matrices of native heart valves. The scaffolds showed anisotropic bending moduli (in both dry and hydrated statuses) depending on the loading directions (lower in the With Curvature direction than in the Against Curvature direction), which mimicked the characteristic behavior of the native heart valves. The interactions between cardiosphere‐derived cells and the scaffolds were characterized by multiphoton microscopy, and relatively similar cell distributions were observed on different layers (a cell density of 3,000–4,000 mm −3 and a migration depth of 0.3–0.4 mm). The trilayer scaffold has represented a forwarding step from the previous studies, in attempting to better replicate a native heart valve structurally, mechanically, and biologically.