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Enhanced bone formation onto the bone surface using a hydroxyapatite/collagen bone‐like nanocomposite
Author(s) -
Hiratsuka Taizo,
Uezono Masayoshi,
Takakuda Kazuo,
Kikuchi Masanori,
Oshima Sho,
Sato Taira,
Suzuki Shoichi,
Moriyama Keiji
Publication year - 2020
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34397
Subject(s) - materials science , periosteum , bone formation , biomedical engineering , bone healing , multinucleate , nanocomposite , composite material , anatomy , pathology , medicine , endocrinology
Abstract The process of bone formation onto the bone surface using a hydroxyapatite/collagen bone‐like nanocomposite (HAp/Col) was investigated. Immersion tests were performed to evaluate the impact of pH on the degradation of the specimens in an aqueous environment. The specimens were soaked in aqueous solutions of pH 4.0, 5.0, and 7.0. Using standardized images, the top‐view areas of the specimens were measured. Animal experiments were performed to investigate the bone formation process onto the bone surface. The specimens were placed under the rat calvarial periosteum, and μCT image analysis and histological observation were performed on samples harvested on postoperative Days 3, 5, and 7. In all experiments, β‐tricalciumphosphate (β‐TCP) was adopted as the control. HAp/Col turned to gel in acidic environments below pH 5.0. In contrast to the β‐TCP, the HAp/Col specimens placed under the periosteum expanded and attained a hollow structure with a gel‐filled center, accompanied by larger volume of new bone and appearance of TRAP‐positive multinucleated cells on postoperative Day 5. Therefore, HAp/Col can enhance bone formation onto the bone surface via induction of TRAP‐positive multinucleated cells, and may have clinical applications.