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Fabrication and characterization of the 3D‐printed polycaprolactone/fish bone extract scaffolds for bone tissue regeneration
Author(s) -
Heo SeongYeong,
Ko SeokChun,
Oh GunWoo,
Kim Namwon,
Choi IlWhan,
Park Won Sun,
Jung WonKyo
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34286
Subject(s) - polycaprolactone , materials science , scaffold , osteopontin , biomedical engineering , osteocalcin , regeneration (biology) , ultimate tensile strength , bone tissue , alkaline phosphatase , bone morphogenetic protein 2 , composite material , chemistry , microbiology and biotechnology , in vitro , medicine , polymer , biology , biochemistry , enzyme
Fish bone extract (FBE) containing a trioligopeptide (FBP‐KSA, Lys‐Ser‐Ala) isolated from Johnius belengerii could induce osteogenic activities on MC3T3‐E1 pre‐osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three‐dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self‐assembly process. In the physical characteristic and mechanical property tests, the results demonstrated that the fabricated scaffolds have the strut diameter (between 340 and 345 μm), pore size (between 470 and 480 μm), porosity (between 50% and 55%), and tensile properties (Young's modulus: 9.18–9.42 MPa; max tensile strengths 82.3–87.4 MPa) were similar to those of PCL scaffold. In the cell proliferation and osteogenic assay, the results showed that PCL/FBE scaffolds could significantly induce cell proliferation, calcium deposition, and the expression of osteogenic phenotype markers such as alkaline phosphatase, osteopontin, osteocalcin, and bone morphogenetic protein‐2 in the osteoblasts. These results suggest that FBE‐coated PCL scaffolds are promising materials for use in biomedical application to promote bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1937–1944, 2019.