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An antibiofilm coating of 5‐aryl‐2‐aminoimidazole covalently attached to a titanium surface
Author(s) -
Peeters Elien,
Hooyberghs Geert,
Robijns Stijn,
De Weerdt Ami,
Kucharíková Soňa,
Tournu Hélène,
Braem Annabel,
Čeh Katerina,
Majdič Gregor,
Španič Tanja,
Pogorevc Estera,
Claes Birgit,
Dovgan Barbara,
Girandon Lenart,
Impellizzeri Frédéric,
Erdtmann Martin,
Krona Annika,
Vleugels Jef,
Fröhlich Mirjam,
GarciaForgas Jordi,
De Brucker Katrijn,
Cammue Bruno P.A.,
Thevissen Karin,
Van Dijck Patrick,
Vanderleyden Jozef,
Van der Eycken Erik,
Steenackers Hans P.
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34283
Subject(s) - biofilm , osseointegration , titanium , staphylococcus aureus , coating , materials science , biomaterial , covalent bond , implant , microbiology and biotechnology , in vivo , surface modification , biomedical engineering , biophysics , bacteria , chemistry , nanotechnology , biology , organic chemistry , surgery , medicine , genetics , metallurgy
Biofilms, especially those formed by Staphylococcus aureus , play a key role in the development of orthopedic implant infections. Eradication of these infections is challenging due to the elevated tolerance of biofilm cells against antimicrobial agents. In this study, we developed an antibiofilm coating consisting of 5‐(4‐bromophenyl)‐ N ‐cyclopentyl‐1‐octyl‐1 H ‐imidazol‐2‐amine, designated as LC0024, covalently bound to a titanium implant surface (LC0024‐Ti). We showed in vitro that the LC0024‐Ti surface reduces biofilm formation of S. aureus in a specific manner without reducing the planktonic cells above the biofilm, as evaluated by plate counting and fluorescence microscopy. The advantage of compounds that only inhibit biofilm formation without affecting the viability of the planktonic cells, is that reduced development of bacterial resistance is expected. To determine the antibiofilm activity of LC0024‐Ti surfaces in vivo , a biomaterial‐associated murine infection model was used. The results indicated a significant reduction in S. aureus biofilm formation (up to 96%) on the LC0024‐Ti substrates compared to pristine titanium controls. Additionally, we found that the LC0024‐Ti substrates did not affect the attachment and proliferation of human cells involved in osseointegration and bone repair. In summary, our results emphasize the clinical potential of covalent coatings of LC0024 on titanium implant surfaces to reduce the risk of orthopedic implant infections. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1908–1919, 2019.