Premium
The effects of substrate‐mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization
Author(s) -
Hu WeiWen,
Chen TunChi,
Tsao ChiaWen,
Cheng YuChe
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34253
Subject(s) - runx2 , duty cycle , stimulation , stromal cell , materials science , mineralization (soil science) , biomedical engineering , alkaline phosphatase , biophysics , microbiology and biotechnology , voltage , chemistry , medicine , endocrinology , biology , enzyme , electrical engineering , biochemistry , engineering , organic chemistry , nitrogen
To explore the effect of electrical stimulation (ES) on osteogenesis, a polypyrrole (PPy)‐made electrical culture system was developed to provide a direct‐current electric field (DCEF). This DCEF device was applied to treat differentiated rat bone marrow stromal cells (rBMSCs) once in different stages of osteo‐differentation to investigate its temporal effects. The mineralization results showed that the DCEF treatment not only accelerated cell differentiation but also promoted the saturation levels, and the ES on day 8 was the group demonstrated the optimal result. The gene regulation analysis indicated that the DCEF treatment immediately increased the levels of genes related to osteo‐differentiation, especially Runx2. Because Runx2 is a crucial transcriptional factor of osteogenesis, the ES‐caused improvement of mineralization was likely contributed by the extension of its expression. Further, different ES modes were investigated of their efficacy on bone matrix deposition. Square waves with different parameters including frequency, offset, amplitude, and duty cycle were systematically examined. In contrast to constant voltage, square waves demonstrated periodical changes of current through substrate to significantly improve mineralization, and the efficiencies highly depended on both frequency and intensity. Through this comprehensive study, DCEF treating condition was optimized, which should be beneficial to its application on osteogenesis promotion. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1607–1619, 2019.