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Application of alginate microbeads as a carrier of bone morphogenetic protein‐2 for bone regeneration
Author(s) -
Lee Yun Hwan,
Lee ByungWoo,
Jung Yun Chan,
Yoon ByungIl,
Woo HeungMyong,
Kang ByungJae
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34119
Subject(s) - bone morphogenetic protein 2 , regeneration (biology) , biomedical engineering , bone morphogenetic protein , mesenchymal stem cell , materials science , alkaline phosphatase , chemistry , microbiology and biotechnology , medicine , biology , biochemistry , enzyme , in vitro , gene
Bone morphogenetic protein‐2 (BMP‐2) is commonly used to enhance bone regeneration. The potential of BMP‐2 for bone regeneration varies according to the concentration and release kinetics on the implanted site. Therefore, it is important to determine appropriate carriers of BMP‐2. However, no optimal delivery vehicles have been identified. In the present study, we used alginate microbeads as a delivery vehicle for BMP‐2. Alginate microbeads can be implanted onto the disease site through surgery or injection. The objective of this study was to evaluate that the osteoinductive properties of BMP‐2 are effective in alginate microbeads as a carrier. In this study, the release kinetics of BMP‐2 in alginate microbeads was evaluated using an enzyme‐linked immunosorbent assay. BMP‐2 released from alginate microbeads induced high alkaline phosphatase activity in canine adipose tissue‐derived mesenchymal stem cells. Injection of alginate microbeads with BMP‐2 into mouse subcutaneous tissue, as well as surgical implantation into the 5‐mm circular calvarial defects in rats, was conducted and the results showed extensive new bone formation. In conclusion, alginate microbeads can be utilized as an effective BMP‐2 delivery vehicle for use in orthopedic surgery and as an injectable vehicle for a minimally invasive therapy. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 286–294, 2019.