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Evaluation of the temporary effect of physical vapor deposition silver coating on resistance to infection in transdermal skin and bone integrated pylon with deep porosity
Author(s) -
Shevtsov Maxim A.,
Yudintceva Natalia M.,
Blinova Miralda I.,
Voronkina Irina V.,
Suslov Dmitriy N.,
Galibin Oleg V.,
Gavrilov Dmitriy V.,
Akkaoui Michael,
Raykhtsaum Grigoriy,
Albul Andrey V.,
Pitkin Emil,
Pitkin Mark
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34108
Subject(s) - coating , materials science , biomedical engineering , implant , mesenchymal stem cell , in vivo , periprosthetic , dermis , porosity , staphylococcus epidermidis , staphylococcus aureus , medicine , surgery , pathology , composite material , bacteria , biology , arthroplasty , genetics , microbiology and biotechnology
Periprosthetic infection via skin‐implant interface is a leading cause of failures and revisions in direct skeletal attachment of limb prostheses. Implants with deep porosity fabricated with skin and bone integrated pylons (SBIP) technology allow for skin ingrowth through the implant's structure creating natural barrier against infection. However, until the skin cells remodel in all pores of the implant, additional care is required to prevent from entering bacteria to the still nonoccupied pores. Temporary silver coating was evaluated in this work as a means to provide protection from infection immediately after implantation followed by dissolution of silver layer in few weeks. A sputtering coating with 1 µm thickness was selected to be sufficient for fighting infection until the deep ingrowth of skin in the porous structure of the pylon is completed. In vitro study showed less bacterial ( Staphylococcus aureus, Staphylococcus epidermidis , and Pseudomonas aeruginosa ) growth on silver coated tablets compared to the control group. Analysis of cellular density of MG‐63 cells, fibroblasts, and mesenchymal stem cells (MSCs) showed that silver coating did not inhibit the cell growth on the implants and did not affect cellular functional activity. The in vivo study did not show any postoperative complications during the 6‐month observation period in the model of above‐knee amputation in rabbits when SBIP implants, either silver‐coated or untreated were inserted into the bone residuum. Three‐phase scintigraphy demonstrated angiogenesis in the pores of the pylons. The findings suggest that a silver coating with well‐chosen specifications can increase the safety of porous implants for direct skeletal attachment. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 169–177, 2019.