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Wnt and Notch signaling pathways in calcium phosphate‐enhanced osteogenic differentiation: A pilot study
Author(s) -
Wang Yu,
Pan Jianwei,
Zhang Yiqun,
Li Xiang,
Zhang Zhen,
Wang Peng,
Qin Zhifan,
Li Jihua
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34105
Subject(s) - wnt signaling pathway , dkk1 , notch signaling pathway , microbiology and biotechnology , mesenchymal stem cell , signal transduction , wnt3a , stromal cell , biology , cancer research
Calcium phosphate (CaP) ceramics are being increasingly used to repair critical bone defect for biocompatibility, biosafety, porous structure and osteoinduction. Wnt and Notch signaling pathways are conservative and classic signaling pathways regulating osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs). In this study, we evaluated the expression of Wnt, Notch signal pathways and osteogenic related genes of BMSCs with and without Wnt pathway inhibitor DKK1 on CaP ceramics in vitro by real‐time RT‐PCR. Also, we evaluated new bone formation after implantation of CaP ceramics in vivo. The in vitro culture displayed the expression of Wnt, Notch signaling pathways and osteogenic related genes was increased at first and then declined. After adding DKK1 to the culture, the expression was decreased. The in vivo implantation showed significant bone formation on CaP ceramics. These findings reveal that Wnt and Notch signaling pathways play important roles in the CaP‐enhanced osteogenic differentiation but the mechanism of synergistic effect needs to be further explored. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 149–160, 2019.