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Silk fibroin produced by transgenic silkworms overexpressing the Arg‐Gly‐Asp motif accelerates cutaneous wound healing in mice
Author(s) -
Baba Atsunori,
Matsushita Shigeto,
Kitayama Kasumi,
Asakura Tetsuo,
Sezutsu Hideki,
Tanimoto Akihide,
Kanekura Takuro
Publication year - 2019
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34098
Subject(s) - fibroin , granulation tissue , wound healing , neovascularization , microbiology and biotechnology , in vitro , wound closure , bombyx mori , materials science , angiogenesis , medicine , chemistry , silk , pathology , biology , surgery , cancer research , biochemistry , composite material , alternative medicine
Abstract We investigated the effect of silk fibroin (SF) on wound healing in mice. SF or an amorphous SF film (ASFF) prepared from silk produced by the wild‐type silkworm Bombyx mori (WT‐SF, WT‐ASFF) or by transgenic worms that overexpress the Arg‐Gly‐Asp (RGD) sequence (TG‐SF, TG‐ASFF) was placed on 5‐mm diameter full‐thickness skin wounds made by biopsy punch on the back of 8–12 week‐old BALB/c mice. Each wound was covered with WT‐ASFF and urethane film (UF), TG‐ASFF plus UF, or UF alone (control). Wound closure, histological thickness, the area of granulation tissue, and neovascularization were analyzed 4, 8, and 12 days later. The effect of SF on cell migration and proliferation was examined in vitro by scratch‐ and MTT‐assay using human dermal fibroblasts. Wound closure was prompted by TG‐ASFF, granulation tissue was thicker and larger in ASFF‐treated wounds than the control, and neovascularization was promoted significantly by WT‐ASFF. Both assays showed that SF induced the migration and proliferation of human dermal fibroblasts. The effects of TG‐ASFF and TG‐SF on wound closure, granulation formation, and cell proliferation were more profound than that of WT‐ASFF and WT‐SF. We document that SF accelerates cutaneous wound healing, and this effect is enhanced with TG‐SF. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 97–103, 2019.