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Behavior of new hydroxyapatite/glucan composite in human serum
Author(s) -
Borkowski Leszek,
Lübek Tomasz,
Jojczuk Mariusz,
Nogalski Adam,
Belcarz Anna,
Palka Krzysztof,
Hajnos Mieczysław,
Ginalska Grazyna
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34082
Subject(s) - biomaterial , swelling , composite number , biomedical engineering , in vivo , fourier transform infrared spectroscopy , scanning electron microscope , materials science , composite material , carrageenan , chemistry , medicine , chemical engineering , biochemistry , biology , microbiology and biotechnology , engineering
Biomaterials for bone tissue regeneration, including polymer‐based composites, are typically evaluated in vitro prior to the clinical trials. However, such composites tested in vivo may behave different due to the specific body conditions. For example, some composites implanted into the tissue acidified due to transient postoperative inflammation may unexpectedly swell which delays the wound healing. Such massive swelling in acidic medium was previously observed for new elastic hydroxyapatite (HAp)/β‐glucan biomaterial. However, in further clinical cases concerning the composite implantation in patients without significant inflammation indicators, no side effects were observed. Therefore, it was reasonable to test the effect of human serum of neutral pH (typical for noninflamed tissues) on the composite parameters, in particular volume changes. Thus, this article shows the characterization of physicochemical parameters of the composite after incubation (5 days) in human serum of neutral pH by means of weight and volume measurement, scanning electron microscopy, X‐ray diffraction, Fourier‐transform infrared spectroscopy, microcomputed tomography, mercury intrusion, and biochemical techniques. Results showed that human serum collected from healthy people caused no uncontrolled changes in weight and volume, porosity and mechanical properties of the composite. Therefore, this suggests the lack of volume change‐related side effects of HAp/glucan composite in bone defects treatment if postoperative inflammation is prevented. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2653–2664, 2018.