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Bacterial nanocellulose‐IKVAV hydrogel matrix modulates melanoma tumor cell adhesion and proliferation and induces vasculogenic mimicry in vitro
Author(s) -
Reis Emily M. dos,
Berti Fernanda V.,
Colla Guilherme,
Porto Luismar M.
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34055
Subject(s) - vasculogenic mimicry , extracellular matrix , self healing hydrogels , mimicry , melanoma , nanocellulose , in vitro , microbiology and biotechnology , cell adhesion , chemistry , cell , cancer research , biology , medicine , biochemistry , cancer , cellulose , metastasis , polymer chemistry , ecology
Vasculogenic mimicry process has generated great interest over the past decade. So far, however, there have been only a few matrices available that allow us to study that process in vitro . Here, we have developed an innovative hydrogel platform with defined composition that mimics the structural architecture and biological functions of the extracellular matrix for vasculogenic mimicry of human melanoma cells (SK‐MEL‐28). We chemically immobilized IKVAV peptide on bacterial nanocellulose (BNC) fibers. BNC‐IKVAV hydrogel was found to improve the adhesion and proliferation of SK‐MEL‐28 cells on the top and bottom surfaces. Particularly, the bottom surface of BNC‐IKVAV induced SK‐MEL‐28 cells to organize themselves as well‐established networks related to the vasculogenic mimicry process. Finally, our results showed that not only BNC‐IKVAV but also BNC hydrogels can potentially be used as a three‐dimensional platform that allows the screening of antitumor drugs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2741–2749, 2018.