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Fibrogenic and angiogenic commitments of human induced pluripotent stem cells derived mesenchymal stem cells in connective tissue growth factor‐delivering scaffold in an immune‐deficient mice model
Author(s) -
Xu Ruodan,
DagnaesHansen Frederik,
Wogensen Lise,
Axelsen Susanne Maigaard,
Seliktar Dror,
Chen Menglin
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.34030
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , stem cell , induced pluripotent stem cell , ctgf , growth factor , biology , endothelial stem cell , immunology , embryonic stem cell , in vitro , biochemistry , receptor , gene
Compared to terminal differentiated cells, stem cells play important roles in the maintenance and regeneration, and thus have been intensively researched as the most promising cell based therapy. In order to maximize the effectiveness of stem cell based therapies, it is essential to understand the environmental (niche) signals that regulate stem cell behavior. Recent findings suggest that fibroblasts have a mesenchymal origin and that mesenchymal stem cells (MSCs) demonstrate proangiogenic function, where both fibrogenic and angiogenic activities are associated with connective tissue growth factor (CTGF), a matricellular protein that serves as an essential mediator of skeletogenesis in development and vascular remodeling. Here, for the first time, we demonstrate that upon local delivery of CTGF from a three dimensional (3D) nanocomposite scaffold, human induced pluripotent stem cells derived MSCs can be directed to differentiate toward fibroblasts in a 3D nanocomposite scaffold in female nonobese diabetic CB‐17/Icr‐severe combined immunodeficient mice. The stem cell‐scaffold constructs present not only intriguingly strong fibroblastic commitments but also angiogenic induction in vivo . © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2266–2274, 2018.