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Characterization of PVA/glutaraldehyde hydrogels obtained using Central Composite Rotatable Design (CCRD)
Author(s) -
MorandimGiannetti Andreia de Araújo,
Rubio Samantha Regina,
Nogueira Regina Freitas,
Ortega Fernando dos Santos,
Magalhães Junior Octaviano,
Schor Paulo,
Bersanetti Patrícia Alessandra
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33958
Subject(s) - glutaraldehyde , crystallinity , self healing hydrogels , materials science , differential scanning calorimetry , swelling , glass transition , composite number , chemical engineering , viscosity , polyvinyl alcohol , rheology , refractive index , composite material , polymer chemistry , polymer , chemistry , chromatography , physics , optoelectronics , engineering , thermodynamics
Hydrogels are made from natural or synthetic polymers and, currently, they have many biomedical applications. In this work, the conditions for obtaining a hydrogel with similar physicochemical characteristics to the vitreous humor were defined using polyvinyl alcohol (PVA) and glutaraldehyde (GLUT) as cross‐linker. The concentration of PVA and GLUT were modified, and their effect was analyzed in terms of the refractive index, density, and dynamic viscosity. The hydrogel which was obtained using 3.98% (w/V) of PVA, 3.13 mL (1.57 g) of GLUT in 100 mL, and the initial pH of 7.2 showed similar characteristics to the vitreous humor (density = 1.0174 ± 0.0050 g mL −1 , dynamic viscosity = 3.7425 ± 0.1800 mPa s and refractive index = 1.3410 ± 0.0010). The hydrogels were further investigated by rheological measurements, infrared spectroscopy, differential scanning calorimetry, X‐ray diffraction and determination of swelling degree. The reticulation with GLUT promoted an increase in viscosity and glass transition temperature. On the other hand, it stimulated a decrease in the swelling degree, crystallinity, melting temperature, and intensity of the band related to the ‐OH bond, compared with the PVA without reticulation. The reticulated hydrogel displayed Newtonian behavior and a higher apparent viscosity than the PVA. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1558–1566, 2018.