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Multiscale regeneration scaffold in vitro and in vivo
Author(s) -
Chen Haiping,
Xie Shikun,
Yang Yuanmo,
Zhang Jing,
Zhang Zhuangya
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33926
Subject(s) - scaffold , regeneration (biology) , extracellular matrix , tissue engineering , in vivo , materials science , biomedical engineering , biocompatibility , electrospinning , chemistry , microbiology and biotechnology , composite material , biology , medicine , biochemistry , metallurgy , polymer
Biocompatible scaffolds play an important role in modulating tissue growth. A gelatin and sodium alginate scaffold with a unique structure produced by a combination of 3‐D printing, electrospinning, and vacuum freeze‐drying has been developed for tissue engineering. The scaffold is composed of a macrostructure, a honeycomb microporous surface morphology, and nanofibers. This structure meets the design criteria for an ideal tissue engineering scaffold. The scaffold degrades and has low cytotoxicity. The biocompatibility of the scaffold is improved by the favorable cell–matrix interaction; cells attach to the scaffold well and secrete large amounts of extracellular matrix in vitro . Rats with the scaffold implanted survived without signs of complications and the host cells infiltrated the interior of the scaffold. After 2 months in vivo , the scaffold was vascularized and contained collagen fibers. This multiscale regeneration scaffold may be suitable for tissue engineering because of its unique structure, degradation, mechanical properties, and lower cytotoxicity, which support cell infiltration and growth, and promote vascularization and generation of granulation tissue in vivo . © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1218–1225, 2018.