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Evaluation of three different decontamination techniques on biofilm formation, and on physical and chemical properties of resin composites
Author(s) -
André Carolina Bosso,
dos Santos Andressa,
Pfeifer Carmem Silvia,
Giannini Marcelo,
Girotto Emerson Marcelo,
Ferracane Jack Liborio
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33907
Subject(s) - human decontamination , materials science , sterilization (economics) , biofilm , fourier transform infrared spectroscopy , ethanol , composite material , chloramine t , nuclear chemistry , chemistry , chemical engineering , bacteria , waste management , organic chemistry , biology , monetary economics , engineering , economics , foreign exchange market , genetics , foreign exchange
Objectives This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. Methods Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans . The surface change, after the process, was analyzed with ATR/FTIR and SEM images. The solutions used for decontamination (1% chloramine‐T and 70% ethanol) were analyzed with 1 H‐NMR to identify any resin compounds leached during the process. Results One minute of decontamination was efficient for all three methods tested. Chloramine‐T increased the surface porosity on resin composites, no changes were observed for UV light and 70% ethanol, however, 1 H‐NMR identified leached monomers only when 70% ethanol was used. No chemical change of the materials was found under ATR/FTIR analyses after the decontamination process. Chloramine‐T, with no previous wash, increased the bacterial viability for both resin composites and increased the bacterial metabolism for the resin composite without fluoride. Conclusion UV light had no interference on the resin composites properties tested using 1 min of exposure compared to the other decontamination methods. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 945–953, 2018.