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Enhanced intestinal absorption of curcumin in Caco‐2 cell monolayer using mucoadhesive nanostructured lipid carriers
Author(s) -
Chanburee Sanipon,
Tiyaboonchai Waree
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33884
Subject(s) - curcumin , bioavailability , polyethylene glycol , permeation , polyvinyl alcohol , zeta potential , solubility , particle size , polymer , materials science , peg ratio , chemistry , chromatography , peg 400 , chemical engineering , nuclear chemistry , nanoparticle , nanotechnology , organic chemistry , pharmacology , biochemistry , membrane , medicine , finance , engineering , economics
This study aimed to compare the intestinal permeation of curcumin‐loaded polymer coated nanostructured lipid carriers (NLCs) and uncoated NLCs using the Caco‐2 cell model. The uncoated NLCs were prepared using a warm microemulsion technique, while polymer‐coated NLCs were prepared with the same method but were followed by coating particle surface with polyethylene glycol (PEG) 400 or polyvinyl alcohol (PVA). After lyophilization, all formulations possessed a mean size of <400 nm with a zeta potential of ∼–30 mV and a high entrapment efficacy up to 90%. All NLCs formulation showed significantly improvement in curcumin water solubility, more than 60‐folds as compared to curcumin dispersion. In addition, they could protect curcumin from degradation in basic pH, 90% curcumin remaining after 6 h incubation in culture medium. In vitro permeation studies revealed that PEG–NLCs and PVA–NLCs provided significantly higher apparent permeation coefficient ( P app ) value than uncoated NLCs. Moreover, after 6 months storage at 4 °C in the absence of sunlight, the physical, and chemical stabilities of the lyophilized curcumin‐loaded polymer coated NLCs and uncoated NLCs could be maintained, i.e., the mean particle size and the amount of curcumin showed no significant changes ( p > 0.05) compared to those freshly prepared formulations. Considered overall, polymer coated NLCs are an important strategy to improve the oral bioavailability of curcumin. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 734–741, 2018.