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C ontrolled release of clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects
Author(s) -
Alenezi Ali,
Naito Yoshihito,
Terukina Takayuki,
Prananingrum Widyasri,
Jinno Yohei,
Tagami Tatsuaki,
Ozeki Tetsuya,
Galli Silvia,
Jimbo Ryo
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33844
Subject(s) - calvaria , plga , in vivo , clarithromycin , bone healing , chemistry , x ray microtomography , bone formation , regeneration (biology) , saline , histology , medicine , biomedical engineering , anatomy , endocrinology , pathology , in vitro , biology , biochemistry , microbiology and biotechnology , antibiotics , radiology
This study evaluated the sustained release effect of clarithromycin‐loaded in PLGA microspheres in a rabbit calvaria defect model. Four bone defects (ø5.0) were created in the calvaria of New Zealand White rabbits ( n = 21, n = 7/time point). The defects were randomly designated to four groups. Group 1: No augmentation (sham), Group 2: beta‐tricalcium phosphate (β‐TCP), Group 3: β‐TCP with 0.12 µg clarithromycin, and Group 4: β‐TCP with 6.12 µg PLGA microspheres loaded with 0.12 µg Clarithromycin. After 2, 4, and 12 weeks of healing, bone regeneration was evaluated using micro‐computed tomography (µCT) and histology. Clarithromycin release from PLGA microspheres revealed sustained release for around 4 weeks with ∼50% release during the first week. Histologically, new bone formation was evident at 2 and 4 weeks of healing in all groups and bone formation increased as a function of healing time. At 12 weeks, Group 4 showed significantly higher amount of newly formed bone compared to Group 1. The µCT showed that Group 4 expressed significantly higher bone formation compared to Group 1 at all time points. The in vivo findings showed that β‐TCP with clarithromycin‐loaded microspheres can enhance bone formation in bone defects. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 201–208, 2018.