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Hybrid extracellular matrix design for cartilage‐mediated bone regeneration
Author(s) -
Mikael Paiyz E.,
Kim Hyun S.,
Nukavarapu Syam P.
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33842
Subject(s) - endochondral ossification , chondrogenesis , cartilage , extracellular matrix , materials science , regeneration (biology) , microbiology and biotechnology , biomedical engineering , ossification , mesenchymal stem cell , bone healing , tissue engineering , anatomy , biology , medicine
Recapitulating long bone repair through endochondral ossification (EO) is increasingly becoming a more popular approach. A successful EO Process depends greatly on the establishment of a healthy hypertrophic‐cartilage template (HCT). The aim of this work is to design a hydrogel system, which closely mimics the extracellular matrix of HCT. We examined the combinatorial effect of two commonly used hydrogels for bone and cartilage regeneration strategies, hyaluronan (HA) and fibrin (FB), to induce HCT formation. Hydrogel combinations were evaluated using a clinically relevant cell source, human bone marrow mesenchymal stem cells (hBMSCs). The results establish that with increasing HA (50–90%) the chondrogenic and its subsequent hypertrophy trend improved, with 70:30 HA:FB combination showing the highest and most uniform expression of chondrogenic and hypertrophic stage specific markers. This combination also showed superior support for cell micro‐aggregation and differentiation. Thus, 70:30 HA‐FB matrix demonstrated a healthy formation of chondrogenic and hypertrophic stages with rich stage‐specific ECM components. This study demonstrates that with the appropriate hydrogel design it is possible to develop effective tissue engineering therapies for bone defect repair and regeneration through endochondral ossification by establishing a healthy HCT. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 300–309, 2018.