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Fe 3+ / SeO 4 2 − dual doped nano hydroxyapatite: A novel material for biomedical applications
Author(s) -
Alshemary Ammar Z.,
Engin Pazarceviren Ahmet,
Tezcaner Aysen,
Evis Zafer
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33838
Subject(s) - fourier transform infrared spectroscopy , simulated body fluid , nuclear chemistry , materials science , scanning electron microscope , adsorption , apatite , infrared spectroscopy , vickers hardness test , analytical chemistry (journal) , microstructure , chemistry , chemical engineering , mineralogy , metallurgy , chromatography , organic chemistry , composite material , engineering
Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 3+ )/selenate (SeO 4 2 −) ions into the HA structure (Ca 10‐ x ‐ y Fe y (PO 4 ) 6‐ x (SeO 4 ) x (OH) 2‐ x ‐ y O y ) (Fe‐SeHA) through a microwave refluxing process. The Fe‐SeHA materials were characterized by X‐ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe‐SeHA samples were phase pure at 900°C. FESEM images showed that formation of rod‐like shaped particles was inhibited dramatically with increasing Fe 3+ amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 3+ concentrations. Optical spectra of Fe‐SeHA materials contained broadband over (200–600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 3+ /SeO 4 2 −ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe‐SeHA discs with Ca/P ratio of 1.35–1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe‐SeHA samples significantly increased with increasing Fe 3+ concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB) cell line and the results demonstrated that hFOB cells attached and proliferated faster on the Fe‐SeHA materials compared to pure HA showing that Fe‐SeHA materials were cytocompatible. ALP activity and intracellular calcium of hFOB cells on 1Fe‐SeHA discs were statistically higher than pure HA, suggesting that presence of Fe 3+ ion supported osteogenic differentiation of hFOB cells. Our results suggest that 1Fe‐SeHA (0.2 M Fe 3+ /0.5 MSeO 4 2 −co‐doped HA) material could be considered as a promising candidate material for orthopedic applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 340–352, 2018.