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Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small‐diameter blood vessels
Author(s) -
Pu Lei,
Wu Jian,
Pan Xingna,
Hou Zongliu,
Zhang Jing,
Chen Wenmin,
Na Zhuhui,
Meng Mingyao,
Ni Haiyan,
Wang Liqiong,
Li Yaxiong,
Jiang Lihong
Publication year - 2018
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33827
Subject(s) - decellularization , scaffold , extracellular matrix , sodium dodecyl sulfate , biomedical engineering , tissue engineering , blood vessel , chemistry , materials science , chromatography , biochemistry , medicine , psychiatry
Although detergent‐based decellularization protocols have been widely used to obtain a natural extracellular matrix (ECM) scaffold in tissue engineering, some key challenges still exist. To achieve an optimum natural decellularized scaffold for the construction of tissue‐engineered small‐diameter blood vessels (TEBV), porcine carotid arteries (PCAs) were decellularized by combining sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC) and Triton X‐100 (Triton) in different concentrations. Tissue samples were processed and their histological, biochemical and biomechanical characteristics were investigated. Results showed that only two methods 0.5% (SDS + SDC) and 1% (SDS + SDC) could completely remove of the cellular contents and preserve the native ECM architecture. Furthermore, 1% (SDS + SDC) based methods acquire preferable porosity and suitable mechanical strength. Residual Triton in the ECM scaffold holds intensive cytotoxity. In conclusion, 1%(SDS + SDC) based method can obtain a superior PCAs scaffold for the construction of TEBV. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 619–631, 2018.