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Novel silk protein barrier membranes for guided bone regeneration
Author(s) -
Smeets Ralf,
Knabe Christine,
Kolk Andreas,
Rheinnecker Michael,
Gröbe Alexander,
Heiland Max,
Zehbe Rolf,
Sachse Manuela,
GroßeSiestrup Christian,
Wöltje Michael,
Hanken Henning
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33795
Subject(s) - membrane , silk , biocompatibility , fibroin , regeneration (biology) , materials science , biomedical engineering , chemistry , microbiology and biotechnology , medicine , biology , composite material , biochemistry , metallurgy
Abstract This study assesses the biocompatibility of novel silk protein membranes with and without modification, and evaluates their effect on facilitating bone formation and defect repair in guided bone regeneration. Two calvarian bone defects 12 mm in diameter were created in each of a total of 38 rabbits. Four different types of membranes, (silk‐, hydroxyapatite‐modified silk‐, β‐TCP‐modified silk‐ and commonly clinically used collagen‐membranes) were implanted to cover one of the two defects in each animal. Histologic analysis did not show any adverse tissue reactions in any of the defect sites indicating good biocompatibility of all silk protein membranes. Histomorphometric and histologic evaluation revealed that collagen and β‐TCP modified silk membranes supported bone formation (collagen: bone area fraction p = 0.025; significant; β‐TCP modified silk membranes bone area fraction: p = 0.24, not significant), guided bone regeneration and defect bridging. The bone, which had formed in defects covered by β‐TCP modified silk membranes, displayed a more advanced stage of bone tissue maturation with restoration of the original calvarial bone microarchitecture when compared to the bone which had formed in defects, for which any of the other test membranes were used. Micro‐CT analysis did not reveal any differences in the amount of bone formation between defects with and without membranes. In contrast to the collagen membranes, β‐TCP modified silk membranes were visible in all cases and may therefore be advantageous for further supporting bone formation beyond 10 weeks and preventing soft tissue ingrowth from the periphery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2603–2611, 2017.