Premium
Osteogenic capacity of alkali‐free bioactive glasses. In vitro studies
Author(s) -
Brito Ana F.,
Antunes Brígida,
dos Santos Francisco,
Fernandes Hugo R.,
Ferreira José M. F.
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33771
Subject(s) - bioactive glass , mesenchymal stem cell , von kossa stain , calcium , alkali metal , in vitro , biomedical engineering , materials science , alkaline phosphatase , chemistry , dentistry , microbiology and biotechnology , biochemistry , biology , medicine , organic chemistry , composite material , enzyme
The high alkali content bioactive glasses commonly used to regenerate bone in dental, orthopedic, and maxillofacial surgeries induce some cytotoxicity in surrounding tissues. The present study aims the ability of some alkali‐free bioactive glasses compositions, recently developed by our research group, to stimulate human mesenchymal stem cells (hMSCs) differentiation into osteoblasts in comparison to 45S5 Bioglass ® . The obtained results demonstrated that alkali‐free bioactive glasses possess higher stimulating towards differentiation of hMSCs in comparison to the control 45S5 Bioglass ® . The von Kossa assay demonstrated that all bioactive glasses studied were able to induce the appearance of calcium deposits even when the cells were cultured in DMEM, proving that these biomaterials per se induce hMSCs cell differentiation. It was also observed that in both cell culture medium used (DMEM, and osteogenesis differentiation medium) alkali‐free bioactive glasses clearly induced the appearance of more calcium deposits than the 45S5 Bioglass ® , indicating their greater ability to induce cell differentiation. In summary, these results indicate that alkali‐free bioactive glasses are a safe and valid alternative to 45S5 Bioglass ® . © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2360–2365, 2017.