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Early tissue formation on whole‐area osteochondral defect of rabbit patella by covering with fibroin sponge
Author(s) -
Hirakata Eiichi,
Tomita Naohide,
Tamada Yasushi,
Suguro Toru,
Nakajima Masaaki,
Kambe Yusuke,
Yamada Keisuke,
Yamamoto Koji,
Kawakami Masahiro,
Otaka Akihisa,
Okumura Hideo,
Suzuki Shigehiko
Publication year - 2016
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33656
Subject(s) - fibroin , sponge , anatomy , cartilage , hyaline cartilage , materials science , patella , tissue engineering , biomedical engineering , articular cartilage , pathology , biology , medicine , osteoarthritis , composite material , alternative medicine , silk , botany
Large osteochondral defects have been difficult to repair via tissue engineering treatments due to the lack of a sufficient number of source cells for repairing the defect and to the severe mechanical stresses affecting the replacement tissue. In the present study, whole‐area osteochondral defects of rabbit patella were covered and wrapped with a fibroin sponge containing chondrocytes, with or without Green Fluorescent Protein (GFP) transgenic marking, on the surface facing the osteochondral defect. Five of eight osteochondral defects that were covered with the chondrocyte‐seeded fibroin sponges showed hyaline cartilage‐like repair containing no fibroin fragments at 6 weeks after surgery. The repaired tissue showed a layer formation, which showed intensive safranin‐O and toluidine blue staining, and which showed positive type II collagen immunostaining. The average surface coverage of the repaired cartilage was 53%. On average, 48% of the cells in the repaired tissue were derived from GFP transgenic chondrocytes, which had been seeded in the fibroin sponge. The fibroin‐sponge covering had the potential to allow the early repair of large osteochondral defects. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1474–1482, 2016.