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Repair of critical size defects using bioactive glass seeded with adipose‐derived mesenchymal stem cells
Author(s) -
Saçak Bülent,
Certel Furkan,
Akdeniz Zeynep D.,
Karademir Betül,
Ercan Feriha,
Özkan Naziye,
Akpinar İhsan Nuri,
Çelebiler Özhan
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33634
Subject(s) - calvaria , bioactive glass , mesenchymal stem cell , adipose tissue , regeneration (biology) , bone healing , resorption , dentistry , biomedical engineering , materials science , chemistry , medicine , anatomy , pathology , microbiology and biotechnology , biology , in vitro , biochemistry
Bioactive glass has been demonstrated as a biocompatible bone substitute. However bone healing process can be prolonged due to late resorption of the material. Adipose derived stem cells (ASC) have osteogenic differentiation potential and hence can be a cell source for bone regeneration. The aim of this study was to test whether combination of bioactive glass with ASCs would enhance bone regeneration. Following creation of critical sized defects on the calvaria of 32 Wistar rats, the animals were randomly divided into four groups: Group C (control): Defects were left untreated; Group G: Defects were covered with autologous bone graft; Group BG: Defects were filled with bioactive glass; Group BG/ASC: Defects were filled with bioactive glass seeded with ASCs. The defect size was significantly greater in Group C compared to all other groups. Bone density was significantly lower in Group C compared to Group G and Group BG/ASC. Bone regeneration score of Group C was significantly lower than other groups. Group BG/ASC demonstrated lamellar bone and havers canal formation. The results of this study demonstrated that bioactive glass implanted with ASC is a biocompatible construct stimulating radiologically and histologically evident bone regeneration similar to autologous bone grafting. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1002–1008, 2017.