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Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa
Author(s) -
Keane Timothy J.,
Dziki Jenna,
Castelton Arthur,
Faulk Denver M.,
Messerschmidt Victoria,
Londono Ricardo,
Reing Janet E.,
Velankar Sachin S.,
Badylak Stephen F.
Publication year - 2017
Publication title -
journal of biomedical materials research part b: applied biomaterials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 108
eISSN - 1552-4981
pISSN - 1552-4973
DOI - 10.1002/jbm.b.33556
Subject(s) - decellularization , extracellular matrix , scaffold , in vivo , tissue engineering , in vitro , microbiology and biotechnology , biomedical engineering , cell , pathology , chemistry , materials science , medicine , biology , biochemistry
Gastrointestinal pathologies, injuries, and defects affect millions of individuals each year. While there are diverse treatment options for these individuals, no ideal solution exists. The repair or replacement of gastrointestinal tissue, therefore, represents a large unmet clinical need. Biomaterials derived from extracellular matrix (ECM) scaffolds have been effectively used to repair or replace numerous tissues throughout the body in both preclinical and clinical studies. Such scaffolds are prepared from decellularized tissues, and the biochemical, structural, and biologic properties vary depending upon the source tissue from which the ECM is derived. Given the potential benefit of a site‐specific ECM scaffold for some applications, the objective of this study was to prepare, characterize, and determine the in vitro and in vivo cell response to ECM derived from porcine colon. Results of this study show that porcine colon can be effectively decellularized while retaining biochemical and structural constituents of the source tissue. Two forms of colonic ECM, scaffold and hydrogel, were shown to be cell friendly and facilitate the polarization of macrophages toward an M2 phenotype both in vitro and in vivo . © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 291–306, 2017.